Abstract
ABSTRACTNonreducing sugars such as sucrose, raffinose, myo‐inositol, pinitol and galactinol were separated on a polyamine‐bonded polymer gel (NH2 type) column using acetonitrile‐water as mobile phase. Sugars in the eluate were detected by periodate oxidation and the subsequent derivation of guanidine to fluorescent compounds. Detection limits were: sucrose 2.5, D‐glucose 2.5 and pinitol 4 ng. This highly sensitive method was more accurate and rapid for determining sugar contents in immature soybean seeds, sweet corn kernels and chinese mustard leaves than the conventional method using refractive index detection. Also, this method reduced the injection volumes of extracts to the column and allowed to lengthen the column life.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
