SU-E-I-63: Evaluation of Liver Metabolites with Choline-Containing Compounds in Non-Alcoholic Fatty Liver Disease Rat Model

Abstract

Purpose: The objective of this research is to characterize the metabolic changes in a fatty liver rat model induced by a high-fat (HF) diet. Methods: To test coil performance, a lipid standard phantom containing canola oil, and a choline (Cho) standard phantom containing 6 mM phosphocholine chloride, copper sulfate (CuSO4, 3 mM) and trimethylsilyl-propionic acid (TSP, 1 mM) in sodium chloride (NaCl, 34 mM) were prepared in a cylindrical bottle. The calibration phantom contained an identical 6 mM phosphocholine chloride, CuSO4, NaCl, and TSP solution, prepared in a conical tube. Male Sprague-Dawley rats (n=12) fed the HF diet, with weight-matched normal-chow (NC) diet rats were housed with ad libitum access to water. Examinations were performed on a 3.0 T scanner (Achieva Tx 3.0 T; Philips Medical Systems, Netherlands). Localized point-resolved spectroscopy sequence was used for periodically acquiring liver spectrum at fortnightly intervals with the following parameters: repetition time/echo time = 1500/35 ms; the number of signal averages = 64. The water signal of each volume of interest was suppressed by variable pulse power and optimized relaxation delays applied before the scan. Cho T1 measurements (TE, 40 ms; TR, 600–1400 ms) and Cho T2 measurements (TR, 6000 ms; TE, 40–220) were obtained. Results: The Cho concentration in HF diet rats was to be 6.0±2.7 mM; the Cho concentration in NC rats was 5.4±1.3 mM. The total lipid of HF diet rats at 4 weeks (8.3±2.2×103) and 8 weeks (8.2±2.1×103) was similar values. These values were higher than those of NC rats (1.7±0.6×103). The total saturated fatty acid of HF diet rats at 4 weeks (14.7±4.5) and 8 weeks (17.2±4.7) were similar. Conclusion: In this study, we show the feasibility of accurately measuring hepatic lipids, with a correction of relaxation time, and a practical external standard method. This study was supported by grant (2012-007883 and 2014R1A2A1A10050270) from the Mid-career Researcher Program through the NRF funded by Ministry of Science. In addition, this study was supported by the Industrial R&D of MOTIE/KEIT (10048997, Development of the core technology for integrated therapy devices based on real-time MRI-guided tumor tracking).