Abstract
The characteristics of sucrose uptake into the symplast of phloem tissue discs harvested from fresh, actively-growing carrot storage roots are described. Sucrose uptake exhibited a curvilinear response with increasing sucrose concentration. The inhibitors p-chloromercuribenzenesulphonic acid (PCMBS) and carbonyl cyanide m-chlorophenylhydrazone (CCCP) decreased uptake and resulted in solely linear relationships between uptake and sucrose concentration. These results suggest that active carrier-mediated transport occurs at the plasmalemma in addition to a diffusive mechanism. The former saturates at a lower concentration (approximately 20 mM) than the latter which does not saturate below 100 mM
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