Abstract
Substrate levels of sucrose were shown to reduce chlorophyll synthesis in carrot tissue culture strain CRT1 but not in strain CRT2. In CRT1 the effect was shown to be a suppression of greening specifically by sucrose rather than a reducing sugar requirement for chlorophyll synthesis. In CRT1 sucrose caused both a reduction in chloroplast numbers per cell and a suppression of lamellar development in plastids. This effect on chloroplast structure was consistent with the observed reduced photosynthetic efficiency (micromoles CO2 per hour per mg chlorophyll) of CRT1 calluses grown on sucrose.
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