Abstract
The objective of this work was to study the activity of sucrose metabolizing enzymes in extracts of cell suspension cultures of Bauhinia forficata Link, Curcuma zedoaria Roscoe and Phaseolus vulgaris L. Invertase pathway was identified in the three studied species. Sucrose synthase pathway was also responsible for sucrose metabolism in Curcuma zedoaria and Phaseolus vulgaris cells. Activity values higher than 300 nmol min-1 mg-1 of protein were found for acid and neutral invertases, UDPglucose pyrophosphorylase and phosphoglucomutase in the cell extract of the three plant species. Sucrose synthase showed low activity in Bauhinia forficata cells. As sucrose concentration in the culture medium decreased, sucrose synthase activity increased in C. zedoaria and P. vulgaris cells. The glycolytic enzymes activity gradually reduced at the end of the culture period, when carbohydrate was limited.
Highlights
Sucrose is the most common sugar translocated in plants and it often represents over 95% of the dry weight of translocated materials (Zimmermann & Ziegler, 1975)
Even though carbohydrates are of prime importance for cell growth, maintenance and differentiation in vitro, the fundamental aspects of carbon utilization and metabolism in cell and tissue cultures have yet to be fully understood (Romano et al, 1995; Vu et al, 1995)
Cultures were maintained at 25±2°C under a 16 hour photoperiod (50.8±6.6 mmol m-2 s-1) by using cool-white fluorescent lamps, General Electric/40W (B. forficata and P. vulgaris) or under darkness (C. zedoaria)
Summary
Sucrose is the most common sugar translocated in plants and it often represents over 95% of the dry weight of translocated materials (Zimmermann & Ziegler, 1975). It has been widely used as the (1)Accepted for publication on October 24, 2000. Even though carbohydrates are of prime importance for cell growth, maintenance and differentiation in vitro, the fundamental aspects of carbon utilization and metabolism in cell and tissue cultures have yet to be fully understood (Romano et al, 1995; Vu et al, 1995). Plant cell and tissue culture metabolize sucrose to provide energy and carbon skeletons for every metabolic process that the cell will conduct.
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