Abstract
Objective: To study the adherence of Streptococcus mutans biofilm after induction with sucrose and xylitol. Material and Methods: Laboratory experimental study incorporating posttest-only control group design. S. mutans biofilm was generated for 24 hours at a temperature of 37°C using BHIB with 5% sucrose and BHIB with 1% xylitol. An adherence assay was conducted in accordance with the method applied previously. The quantity of adhered bacteria was measured by means of a spectrophotometer at 570 nm. The data were presented as mean and standard deviation. Results: A biofilm induced with sucrose has a higher adherence level (0.9294 ± 0.0431) compared with one induced with xylitol (0.5095 ± 0.0392). Sucrose induces adherence levels by increasing glucan binding protein and glucosyltransferase of the bacteria, whereas xylitol will inhibit the glycolysis process of the bacteria. Conclusion: The adherence of sucrose-induced S. mutans biofilm is higher than that of xylitol-induced S. mutans biofilm.
Highlights
According to Indonesia’s National Basic Health Research Department (RISKESDAS), in 2018, the national prevalence of oral disease was 57.6%, with a DMF-T index of 8.43
S. mutans biofilm was generated for 24 hours at a temperature of 37°C using BHIB with 5% sucrose and BHIB with 1% xylitol
Several bacteria are involved in caries development, but the predominant strain is Streptococcus mutans, which demonstrates the ability to metabolize carbohydrate as a food source
Summary
According to Indonesia’s National Basic Health Research Department (RISKESDAS), in 2018, the national prevalence of oral disease was 57.6%, with a DMF-T index of 8.43. Such an elevated rate requires unprecedented promotive and preventive action on the part of health workers and dentists. Caries are the result of the demineralization of the teeth caused by metabolic activity within the bacterial biofilm covering its surface. S. mutans produces soluble glucan, while insoluble glucan is derived from sucrose metabolism. This correlates with bacterial attachment, matrix formation and sucrose fermentation that forms lactic acid in the biofilm. Biofilm adherence is one of the main problems currently subject to research, which is being conducted as a means of promoting an understanding of how bacterial colonization occurs both within and outside the oral cavity [4]
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