Sucrose analysis in leaf tissue of two contrasted rubber tree clones under abiotic and biotic stresses

Abstract

The sucrose content in rubber leaf is assumed to differ between genotypes and environmental conditions. We studied the relation of sucrose-content and -concentration between high- and low- latex metabolism clones, such as PB 260 and PR 300, respectively under biotic- and abiotic-stresses (white root disease and tapping panel dryness). The experimental design was a complete randomized design of two treatments (by genotypes and by stress conditions) with five replicates. Sucrose analysis was performed using the anthrone colourimetric modified method at 627 nm of spectrophotometry. Statistical analysis used the Student Newman-Keuls test of ANOVA at 5% significant level. The different types of latex metabolism clones had not affected the value of sucrose-content and -concentration in leaf tissue. By contrast, the results showed that WRD-affected trees of PB 260 clone (24.45 mg g−1) had significantly the highest sucrose content compared with healthy- (15.53 mg g−1) and TPD-affected trees (13.42 mg g−1). A similar trend was equally observed in TPD- (16.33 mg g−1), healthy- (14.51 mg g−1) and WRD-affected trees (14.52 mg g−1) of PR 300 clone. The sucrose-content and -concentration in PB 260 clone at WRD-affected trees were the highest than the others.