Successive determination of urinary bilirubin and creatinine employing simultaneous injection effective mixing flow analysis

Abstract

A novel four-channel simultaneous injection effective mixing flow analysis (SIEMA) system has been assembled for successive determination of bilirubin and creatinine in urinary samples. The chemical variables and physical parameters in the flow system were optimized for the enhancement of successive analytical performances. The interferences from urine matrices on the determination of bilirubin and creatinine were eliminated to dilute urine samples. The calibration graphs with the optimum conditions were achieved to be in 0.024–5.0mgL−1 for bilirubin and 2–100mgL−1 for creatinine. The relative standard deviations (RSDs) at 3mgL−1 of bilirubin and at 50mgL−1 of creatinine for 11 runs were 1.5 and 1.0%, respectively. The limits of detections (3σ of blank) for bilirubin and creatinine were 7µgL−1 and 0.6mgL−1, respectively. The sample throughput for stepwise detection was 22h−1. The proposed method was applied to the successive determination of bilirubin and creatinine in urine samples.