Succession of protease activity in seawater and bacterial isolates during starvation in a mesocosm experiment

Abstract

Protein biodegradation in the marine environment is caused by proteases derived from various organisms, including bacteria, which are considered to be a major source of these enzymes. We investigated the succession of bacterial proteases in seawater to determine the variation in protease activity over time. The potential activities of proteolytic enzymes in stored seawater and isolated bacteria were studied using 19 different synthetic oligopeptide substrates for aminopeptidase, trypsin, chymotrypsin and elastase. In time-course experiments carried out over 112 d, aminopeptidase activity increased, whereas trypsin activity decreased over time. Amino peptidase activity was mainly found in unfiltered seawater containing bacterial cells, whereas trypsin activity was mainly found in 0.2 μm seawater filtrates. Individual bacterial isolates showed different proteolytic properties but all exhibited aminopeptidase activity. Members of the Gammaproteobacteria and Bacteroidetes showed high trypsin and chymotrypsin activities. Based on these results, we conclude that protein degradation in seawater occurs via the combined action of various bacterial proteases.