Abstract
Psuedopregnancy for embryo transfer (ET) is usually induced in rats by mating with vasectomized males. Previously, we successfully induced pseudopregnancy using sonic vibration instead (Easy-ET method). The transferred embryos developed normally. Conventionally, stimulation is performed 7 × 30 s with 5 min intervals at the day before ET. However, this protocol is time-consuming because it imitates natural mating behavior. Here, we investigated pseudopregnancy induction with shorter stimulation times. Stimulation was performed 2 × 30 s, with 30 s intervals at the proestrus stage at the day before ET. Of the transferred pronuclear or two-cell embryos, 43% or 62% developed normally, respectively. Furthermore, 67% or 68% of transferred pronuclear or two-cell embryos in rats at estrus stage stimulated on the day of ET developed normally, respectively. Pseudopregnancy was successfully induced with shorter stimulation. Furthermore, this protocol may be used to perform a single-day stimulation and ET operation at the estrus stage.
Highlights
Psuedopregnancy for embryo transfer (ET) is usually induced in rats by mating with vasectomized males
Pseudopregnancy is induced in females at the proestrus stage by mating them with vasectomized males on the day before ET is performed
In the conventional Easy-ET method, pseudopregnancy is induced in females at the proestrus stage by sonic vibration for 30 s per stimulation 7 times in 5 min intervals on the day before ET
Summary
Psuedopregnancy for embryo transfer (ET) is usually induced in rats by mating with vasectomized males. 67% or 68% of transferred pronuclear or two-cell embryos in rats at estrus stage stimulated on the day of ET developed normally, respectively. We successfully induced pseudopregnancy in female rats by artificial stimulation using sonic vibration instead of vasectomized males (the so-called Easy-ET method)[23]. In the conventional Easy-ET method, pseudopregnancy was induced in females at the proestrus stage by stimulation with sonic vibration for 30 s performed 7 times at 5 min intervals one day before ET. This protocol is time consuming because it is designed to imitate natural mating behavior. We studied the possibility of inducing pseudopregnancy with a shortened period of artificial stimulation using the Easy-ET method
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