Successful Hematopoietic Stem Cells (HSC) Mobilization in Patients Who Fail Plerixafor Mobilization for Autologous Stem Cells Transplant (ASCT)

Abstract

Abstract 1913Plerixafor (P) is a novel hematopoietic stem cell (HSC) mobilizing agent approved in 2008 to enhance mobilization of HSC when used in conjunction with granulocyte colony stimulating factor (G-CSF). In spite of the enhanced ability of P to mobilize HSC, 25–35% of patients (pts) fail to mobilize sufficient HSC when P is used with G-CSF. There is dearth of data on mobilization strategies which could be used after P failureWe report our experience of re-mobilizing 24 pts who failed to collect ≥2 × 106 CD34+ cells/ Kg in the first mobilization attempt despite use of P. The clinical characteristics of these 24 pts are shown in Table 1. The median age of the pts was 61 years (range, 31 –70) and 14 (58%) were males. Underlying diagnosis for consideration of autologous stem cell transplant (ASCT) was non-Hodgkin lymphoma (NHL), multiple myeloma (MM) and Hodgkin disease (HD) in 14 (58%), 9 (38%) and 1 (4%) pts, respectively. The median CD34+ cells collected during the first mobilization in these pts using either G-CSF + P (22 pts) or cyclophosphamide (CY) + G-CSF+ P (2 pts) were 0.5 ×106CD34 + cells /Kg (range 0–1.5).Mobilization outcomes of these 24 patients are shown in Table 2. Second mobilization was attempted a median of 22 days (range, 15–106) after the first failed mobilization. The agents used were, granulocyte macrophage colony stimulating factor (GM-CSF) + G-CSF, G-CSF + P, CY + G-CSF and bone marrow harvest in 19, three, one and one pts, respectively. The median CD34+ cell dose collected with the second attempt was 1.1 ×106 CD34 + cells/Kg (range 0–7.2). The median CD34+ cell dose/ Kg collected for the 19 pts who received GM-CSF+ G-CSF and 3 pts who received G-CSF + P were 0.8 ×106 CD34 + cells/Kg (range 0–7.2) and 1.8 ×106 CD34 + cells/Kg (range, 1.4–2.2), respectively; while the two pts who underwent a bone marrow harvest and CY + G-CSF collected 0.9 and 0 × 106CD34+ cells/ Kg, respectively. The cell dose collected when compared to the first attempt was higher in 13 (54%) pts, unchanged in six (25%) pts and lower in five (21%) pts.Third mobilization was attempted in six pts at median of 51 days (range, 34–163) after the first failed mobilization. These pts mobilized a median of 1.1 × 106 CD34+ cells/ Kg (range, 0–6.5). Three of these pts underwent a bone marrow harvest and collected 0.6, 0.7 and 1.5 ×106 CD34+ cells/ Kg. One patient each received GM-CSF + G-CSF, G-CSF + P and CY+ G-CSF and collected 6.5, 4.2 and 0 × 106CD34+ cells/ Kg, respectively. The cell dose collected when compared to the first attempt was higher in four pts (17%), unchanged in one patient (4%) and lower in one patient (4%).Total of 18 pts (75%) collected sufficient cells to undergo ASCT and 17 pts have undergone ASCT. All pts except one received all the collected cells. The median CD34+ cell dose for those who underwent ASCT was 2.8 × 106 CD34+ cells/ Kg (range, 1.4– 6). The median days to white blood cell and platelet engraftment were12 and 16 days post ASCT, respectively. One patient who received 1.4 × 106CD34+ cells/ Kg did not engraft platelets after ASCT.After failing mobilization with P, nine pts (38%) were able to mobilize ≥2 × 106 CD34+ cells/ Kg in a single subsequent attempt which included 7/21 pts who received GM-CSF + G-CSF and 2/4 pts who received G-CSF + P. None of the pts were able to mobilize ≥2 × 106CD34+ cells/ Kg with bone marrow harvest or CY+ G-CSF. Our experience suggests that 75% of the pts who fail primary mobilization with P can be salvaged by a subsequent mobilization with combination of G-CSF with either P or GM-CSF.Table 1Patient CharacteristicsPt CharacteristicsNPercent or (Range)Median Age61 years(31–70)GenderMale1458%Female1042%DiagnosisNHL1458%MM938%HD14%Disease Status at First MobilizationCR/VGPR1146%PR938%Stable312%Refractory14%Chemo regimens prior to mobilization1–21563%>2937%Radiation (XRT)/Radio immunotherapy (RIT) Prior to MobilizationXRT28%RIT14%Mobilization Agent (s) used in First Attempt with PlerixaforG-CSF2292%CY + G-CSF28%Median CD34+ Cell Dose collected at first mobilization0.5 × 106 CD34 + cells/Kg(0–1.5)Table 2Mobilization OutcomesMobilization agentsNApheresisNo. of Patients who collected ≥2×106 cells/kgCollected Cell Dose (×106 cells/kg)YesNoMedianRangeSecond Attempt (N=24)GM-CSF + G-CSF1911860.80–7.2G-CSF+P32011.81.4–2.2CY + G-CSF10100N/ABone Marrow Harvest1N/AN/A00.9N/AThird Attempt (N=6)Bone marrow Harvest3N/AN/A00.70.6–1.5GM-CSF + G-CSF11016.5N/ACY + G-CSF10100.0N/AG-CSF + P11014.2N/A Disclosures:No relevant conflicts of interest to declare.