Abstract
Objective: For men whose spermatogenesis is arrested at the level of round spermatid (R-ST), the round spermatid injection (ROSI) is the only fertility treatment available. However, ROSI is unlikely to be regarded as a certified treatment and many issues still remain to be solved. Main questions among them are low fertilization rate of ROSI and lack of security when using an immature spermatogenic cell. Therefore, we investigated the in vitro culture of R-ST with co-cultured Vero cells, to make them undergo a series of structural and biochemical modifications to improve the pregnancy rate.
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