Successful Diagnosis Of Orthotopic Rat Superficial Bladder Tumor Model By Ultrathin Cystoscopy

Abstract

No AccessJournal of UrologyINVESTIGATIVE UROLOGY1 Feb 2003Successful Diagnosis Of Orthotopic Rat Superficial Bladder Tumor Model By Ultrathin Cystoscopy HIROSHI ASANUMA, TSUNENORI ARAI, KENJI SEGUCHI, SATOKO KAWAUCHI, HIROYUKI SATOH, MAKOTO KIKUCHI, and MASARU MURAI HIROSHI ASANUMAHIROSHI ASANUMA More articles by this author , TSUNENORI ARAITSUNENORI ARAI More articles by this author , KENJI SEGUCHIKENJI SEGUCHI More articles by this author , SATOKO KAWAUCHISATOKO KAWAUCHI More articles by this author , HIROYUKI SATOHHIROYUKI SATOH More articles by this author , MAKOTO KIKUCHIMAKOTO KIKUCHI More articles by this author , and MASARU MURAIMASARU MURAI More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)64000-1AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: In the orthotopic animal bladder tumor model noninvasive evaluation of the tumor establishment and the therapeutic effect has been difficult. To our knowledge we present the first diagnosis of orthotopic rat superficial bladder tumor model by ultrathin cystoscopy. Materials and Methods: The established AY-27 rat bladder carcinoma cell line was transplanted orthotopically into 22 female Fischer F344 rats. A cell suspension containing 4 × 106 AY-27 cells was instilled into the bladder, which had been conditioned with mild acid washing. To evaluate tumor growth serial cystoscopy was performed via the urethra with an ultrathin endoscope (diameter 0.75 mm.) 5 to 14 days after tumor cell inoculation. At intervals after cystoscopic tumor detection the rats were sacrificed for autopsy and histological examination. Results: In all 22 rats the orthotopic bladder tumor was established 7 to 10 days after tumor cell implantation and in most it was superficial. Cystoscopy permitted inspection of the urethra and whole bladder surface. We detected all tumors as broad based papillary mass (minimal lesion 1 mm. or less) and inspected its detailed appearance and accurate location. Conclusions: The orthotopic rat superficial bladder tumor model and the diagnostic procedure by ultrathin cystoscopy would be ideal system for preclinical evaluation of new potential intravesical therapies. References 1 : Magnetic resonance imaging for detecting and treatment monitoring of orthotopic murine bladder tumor implants. J Urol1991; 145: 1297. Link, Google Scholar 2 : Characterization of a novel transplantable orthotopic rat bladder transitional cell tumour model. Br J Cancer1999; 81: 638. Crossref, Medline, Google Scholar From the Department of Urology, School of Medicine and Department of Applied Physics and Physico-Informatics, Faculty of Science and Technology, Keio University, Tokyo and Departments of Urology and Medical Engineering, National Defense Medical College, Tokorozawa, Saitama, Japan© 2003 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited bySatoh H, Morimoto Y, Arai T, Asanuma H, Kawauchi S, Seguchi K, Kikuchi M and Murai M (2018) Intravesical Ultrasonography for Tumor Staging in an Orthotopically Implanted Rat Model of Bladder CancerJournal of Urology, VOL. 177, NO. 3, (1169-1173), Online publication date: 1-Mar-2007.ASANUMA H, ARAI T, MORIMOTO Y, KAWAUCHI S, SATOH H, SEGUCHI K, KIKUCHI M and MURAI M (2018) PHOTODYNAMIC THERAPY WITH PAD-S31, A NEW HYDROPHILIC CHLORIN PHOTOSENSITIZER, IN AN ORTHOTOPIC RAT BLADDER TUMOR MODELJournal of Urology, VOL. 174, NO. 5, (2016-2021), Online publication date: 1-Nov-2005. Volume 169Issue 2February 2003Page: 718-720 Advertisement Copyright & Permissions© 2003 by American Urological Association, Inc.Keywordsratsbladderbladder neoplasmscystoscopesMetricsAuthor Information HIROSHI ASANUMA More articles by this author TSUNENORI ARAI More articles by this author KENJI SEGUCHI More articles by this author SATOKO KAWAUCHI More articles by this author HIROYUKI SATOH More articles by this author MAKOTO KIKUCHI More articles by this author MASARU MURAI More articles by this author Expand All Advertisement PDF downloadLoading ...