Abstract
Capsids of bacteriophage T4 have been dissociated in three different solvents: (1) 6 M guanidine hydrochloride, (2) 6 M guanidine hydrochloride plus 0.1 M β-mercaptoethanol, and (3) 67% acetic acid. Sedimentation equilibrium experiments done in the presence of mercaptoethanol have shown that the capsid subunits can be divided into two classes with molecular weights of approximately 11,000 and 46,000. In the absence of mercaptoethanol, a component of molecular weight 78,000 has been found, indicating that disulfide bonds may be important in stabilizing the phage head structure.
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