Abstract
The Fffilamentous viruses ( fd, M13, f1) are important models for membrane protein assembly and are used extensively as cloning vectors and vehicles for peptide display. The thread-like virion (∼6 nm × 880 nm) comprises a single-stranded DNA genome sheathed by ∼2700 copies of a 50-residue α-helical subunit (pVIII). The average inclination of pVIII subunits from the virion axis has been determined experimentally by polarized Raman microspectroscopy of oriented fdfibers, using the amide I Raman tensors obtained from a peptide single crystal. It is found that the average tilt angle, τ, between the pVIII α-helix and the virion axis falls within the range 13 < τ < 20°.
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