Subunit modification and association in VR1 ion channels

Tamara Rosenbaum,Sharona E Gordon,Mika Awaya

doi:10.1186/1471-2202-3-4

Tamara Rosenbaum, Sharona E Gordon + Show 1 more

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https://doi.org/10.1186/1471-2202-3-4

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Journal: BMC neuroscience	Publication Date: Jan 1, 2002
Citations: 69	License type: cc-by

Affiliation: University of Washington, Seattle University

Abstract

BackgroundThe capsaicin (vanilloid) receptor, VR1, is an agonist-activated ion channel expressed by sensory neurons that serves as a detector of chemical and thermal noxious stimuli.ResultsIn the present study we investigated the properties of VR1 ion channels expressed in Xenopus oocytes. A VR1 subunit with a FLAG epitope tag at the C-terminus was constructed. When examined for size on an SDS gel, VR1-expressing oocytes produced a doublet corresponding to the size of the monomer and a band at about twice the molecular weight of the monomer. A consensus site for N-linked glycosylation was identified in the primary sequence at position 604. In channels in which the putative glycosylation site was mutated from asparagine to serine (N604S), the larger of the two monomer bands could no longer be detected on the gel. Electrophysiological experiments showed these unglycosylated channels to be functional. The high molecular weight band observed on the gel could represent either a dimer or a monomer conjugated to an unknown factor. To distinguish between these possibilities, we coexpressed a truncated VR1 subunit with full-length VR1. A band of intermediate molecular weight (composed of one full-length and one truncated subunit) was observed. This dimer persisted under strongly reducing conditions, was not affected by capsaicin or calcium, and was refractory to treatment with transglutaminase inhibitors.ConclusionsThe persistence of this dimer even under harsh denaturing and reducing conditions indicates a strong interaction among pairs of subunits. This biochemical dimerization is particularly intriguing given that functional channels are almost certainly tetramers.

Highlights

The capsaicin receptor, vanilloid receptor 1 (VR1), is an agonist-activated ion channel expressed by sensory neurons that serves as a detector of chemical and thermal noxious stimuli
The essential functions of nociceptors include the transduction of noxious stimuli into depolarizations that trigger action potentials, conduction of action potentials from peripheral sensory sites to synapses in the central nervous system, and conversion of action potentials into neurotransmitter release at presynaptic terminals, all of which depend on ion channels [6,12–16]
Electrophysiological and biochemical properties of VR1 We expressed VR1 channels in Xenopus oocytes in order to characterize their electrophysiological and biochemical properties

Summary

Introduction

The capsaicin (vanilloid) receptor, VR1, is an agonist-activated ion channel expressed by sensory neurons that serves as a detector of chemical and thermal noxious stimuli. Nociceptors are specialized primary afferent neurons and the first cells in the series of neurons that lead to the sensation of pain [1–8]. The receptors in these cells can be activated by different noxious chemical or physical stimuli [9–11]. The essential functions of nociceptors include the transduction of noxious stimuli into depolarizations that trigger action potentials, conduction of action potentials from peripheral sensory sites to synapses in the central nervous system, and conversion of action potentials into neurotransmitter release at presynaptic terminals, all of which depend on ion channels [6,12–16].

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