Abstract
A 1034 bp cDNA encoding the full length sequence of subunit D of the vacuolar H +-ATPase was cloned from Arabidopsis thaliana. The open reading frame of the cDNA clone vatpD contains 780 bp and codes for a protein of 29.1 kDa with a p I of 9.52. Structural predictions show similarities to subunit γ of the F-ATP synthases. Identity between subunit D of the vacuolar H +-ATPase of A. thaliana and subunits D from other eukaryotic organisms is in the range of 57% ( Bos taurus) to 48% ( Candida albicans). Hybridization of genomic DNA with vatpD indicates the existence of one gene copy of subunit D in A. thaliana. Northern blot hybridization and in situ hybridization showed expression of vatpD in all cell types. The expression of subunit D was not modified by salt stress or abscisic acid treatment in A. thaliana.
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