Abstract
Salmonella, as a zoonotic pathogen, has attracted widespread attention worldwide, especially in the transmission between household pets and humans. Therefore, we investigated the epidemic distribution of dog Salmonella from pet hospitals and breeding base in Xuzhou, Jiangsu Province, China, and used multilocus sequence typing (MLST) and clustered regularly interspaced short palindromic repeats (CRISPRs) to subtype Salmonella isolates. From April 2018 to November 2019, a total of 469 samples were collected from pet hospitals and breeding base, including 339 dog samples and 60 cat samples. S. Kentucky (40.74%) was the most prevalent serotype, but other, such as S. Typhimurium (18.52%) and S. Indiana (18.52%), were also widespread. Eight different sequence type (ST) patterns were identified by MLST and ST198 was the highest proportion of these isolates. CRISPRs analysis showed that 9 different Kentucky CRISPR types (KCTs) was identified from ST198. 48 spacers including 29 (6 News) for CRISPR1 and 19 (4 News) for CRISPR2 that proved the polymorphic of Salmonella genes in samples from different sources. The analysis demonstrated that the common serotypes were widely present in pet hosts in the same area. This analysis shows that CRISPR genes have better recognition ability in the same serotype, which has a positive effect on the traceability of Salmonella and the prevention and treatment of salmonellosis.
Highlights
Salmonella, as a kind of zoonotic bacteria, has caused widespread concern worldwide
From 2006 to 2008, in the United States, a study related to human Salmonella infection and contaminated dry dog and cat food showed that Salmonella
Two molecular typing methods were used. 27 strains of Salmonella from pets were identified with different subtypes using Multilocus sequence typing (MLST); Clustered regularly interspaced short palindromic repeats (CRISPRs) was used to analyze the identical serotypes of S
Summary
Salmonella, as a kind of zoonotic bacteria, has caused widespread concern worldwide. In recent years, the number of pets worldwide has increased dramatically, of which cats and dogs account for 80%. The close contact between humans and pets greatly increases the risk of Salmonella spreading between pets and humans. There are even individual cases that can prove that pet dogs and cats can spread their own Salmonella to humans (Cherry et al 2004; Koehler et al 2006). Molecular detection methods are widely used in the typing of Salmonella strains. This study investigates the genetic diversity and subtype transmission of pet Salmonella between pets and humans in Xuzhou, China. 27 strains of Salmonella from pets were identified with different subtypes using MLST; CRISPR was used to analyze the identical serotypes of S. Kentucky and estimate the potential risk of the same subtypes Salmonella spreading between pets and humans
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