Abstract
AbstractConventional agarose gel electrophoresis (AGE) in Tris/glycine/barbital buffer (TGVB) could classify the common allotype “F” of factor B (BF) into two kinds of subtypes (Fb1 and F). Analysis of mother‐child pairs in paternity cases confirmed inheritance of these suballeles in the same manner as other alleles at the BF locus. The gene frequencies were estimated at 0.0154 for BF*Fb 1 and 0.1492 for BF*F through the study of 325 unrelated individuals. After treatment of serum samples with zymosan, the polymorphic band of BFFb 1 appeared in the Ba fragment, migrating between those of BFF and BFS. A technique of immunoblotting could demonstrate the BF patterns after AGE. This study provides a simple method for the detection of the BF subtypes and their electrophoretic characterization.
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