Subtle Roles of Down Syndrome Cell Adhesion Molecules in Embryonic Forebrain Development and Neuronal Migration.

Manuela D Mitsogiannis,Sonja M Sachse,Eve Seuntjens,Anna Pancho,Dietmar Schmucker,Tania Aerts,Ria Vanlaer,Lut Noterdaeme

doi:10.3389/fcell.2020.624181

Abstract

Down Syndrome (DS) Cell Adhesion Molecules (DSCAMs) are transmembrane proteins of the immunoglobulin superfamily. Human DSCAM is located within the DS critical region of chromosome 21 (duplicated in Down Syndrome patients), and mutations or copy-number variations of this gene have also been associated to Fragile X syndrome, intellectual disability, autism, and bipolar disorder. The DSCAM paralogue DSCAM-like 1 (DSCAML1) maps to chromosome 11q23, implicated in the development of Jacobsen and Tourette syndromes. Additionally, a spontaneous mouse DSCAM deletion leads to motor coordination defects and seizures. Previous research has revealed roles for DSCAMs in several neurodevelopmental processes, including synaptogenesis, dendritic self-avoidance, cell sorting, axon growth and branching. However, their functions in embryonic mammalian forebrain development have yet to be completely elucidated. In this study, we revealed highly dynamic spatiotemporal patterns of Dscam and Dscaml1 expression in definite cortical layers of the embryonic mouse brain, as well as in structures and ganglionic eminence-derived neural populations within the embryonic subpallium. However, an in-depth histological analysis of cortical development, ventral forebrain morphogenesis, cortical interneuron migration, and cortical-subcortical connectivity formation processes in Dscam and Dscaml1 knockout mice (Dscamdel17 and Dscaml1GT) at several embryonic stages indicated that constitutive loss of Dscam and Dscaml1 does not affect these developmental events in a significant manner. Given that several Dscam- and Dscaml1-linked neurodevelopmental disorders are associated to chromosomal region duplication events, we furthermore sought to examine the neurodevelopmental effects of Dscam and Dscaml1 gain of function (GOF). In vitro, ex vivo, and in vivo GOF negatively impacted neural migration processes important to cortical development, and affected the morphology of maturing neurons. Overall, these findings contribute to existing knowledge on the molecular etiology of human neurodevelopmental disorders by elucidating how dosage variations of genes encoding adhesive cues can disrupt cell-cell or cell-environment interactions crucial for neuronal migration.

Highlights

Down Syndrome (DS) Cell Adhesion Molecules (DSCAMs) represent a small group of transmembrane proteins of the immunoglobulin superfamily comprising, in vertebrates, DSCAM and its paralogue DSCAM-like 1 (DSCAML1) (Yamakawa et al, 1998; Agarwala et al, 2001)
At embryonic day (E) 13.5 (n = 5), Dscam was found to be strongly expressed in postmitotic layers of the developing cortex [the marginal zone (MZ) and the preplate (PP)/cortical plate (CP)], and in mantle regions of the ventral telencephalon surrounding the internal capsule (IC), comprising the subpallial corridor dorsally and the globus pallidus ventrally
At E16.5 (n = 5), Dscam expression appeared to have extended to all cortical layers, except for the ventricular zone (VZ), and was found to be robust in deeper cortical plate regions

Summary

Introduction

Down Syndrome (DS) Cell Adhesion Molecules (DSCAMs) represent a small group of transmembrane proteins of the immunoglobulin superfamily comprising, in vertebrates, DSCAM and its paralogue DSCAM-like 1 (DSCAML1) (Yamakawa et al, 1998; Agarwala et al, 2001) These molecules owe their name to the location of human DSCAM within the DS critical region of chromosome 21 (Yamakawa et al, 1998; Schmucker and Chen, 2009), which is considered to be crucially involved in the emergence of cognitive phenotypes associated with DS (Delabar et al, 1993; Korenberg et al, 1994; Belichenko et al, 2009, 2015; Aziz et al, 2018). In Drosophila, a third copy of the Dscam gene results in sensory perception impairments mirroring those found in flies lacking the Fragile X Mental Retardation gene, in which Dscam levels are elevated, and that in the latter animals can be rescued by reducing Dscam expression (Cvetkovska et al, 2013)

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