Substrate Types on Habitat Breeding Embryonic Development and Larval Morphometric of Sea Urchin Tripneustes gratilla (Linnaeus 1758)

Abstract

Hatchery is the main factor that plays an important role in aquaculture sea urchin Tripneustes gratilla (Linnaeus 1758). The quality of sea urchin egg is determined by the absorption of nutrients that carried throughout the gonad development while the quality of nutrients is very likely influenced by the type of food consuming. This research of substrate types on habitat breeding embryonic development and larval morphometric of sea urchin had been done on October - November 2016 at the laboratory of marine, Research Center for Deep Sea, Indonesia Institute of Sciences, Ambon. The Samples taken from three different habitats comprises Hative Besar, Liang and Suli beach. The purpose of this research is to determine the effects of substrate types on habitat breeding embryonic development and larval morphometric of sea urchin. The method that has been used to determine the morphometric was done by measuring the diameter and height of the shell using a digital caliper and total body weight using a digital scale. To observe embryonic development and larval morphometric done by in vitro fertilization through eggs selection then cultured in petri dish at a density of 10ind/ml. The result of this research is the most dominant quantity of embryonic development (72 hours) indicated by culture vessel from Hative Besar meanwhile the least is indicated by Liang. The greatest larval morphometric is indicated by culture vessel from Hative Besar with length and height of larval body further the larval of culture vessel from Hative Besar shown ossification formation.