Abstract
Dimethylethylcholine, dimethyl- n-propylcholine, dimethyl- i-propyl choline, and 2-dimethylaminoethanol are substrates for choline kinase, partially purified from the fat-body of Phormia regina larvae by ammonium sulphate fractionation. Choline analogues and related compounds, which have been previously reported to be effective substitutes for dietary choline, are effective competitive inhibitors of choline phosphorylation. Some additional compounds, triethylcholine, diethylcholine, and 3-trimethylamino-1-propanol, are effective inhibitors of choline phosphorylation yet inadequate as substitutes for dietary choline. Further evidence for separate choline and ethanolamine kinases is provided by studies of both competitive and non-competitive inhibitors. The criteria for an effective inhibitor of ethanolamine phosphorylation are the presence of only one N-alkyl group (except dimethylaminoethanol) and a 2-carbon distance between the nitrogen atom and the hydroxyl group. The I 50 values for the inhibition of choline and ethanolamine kinases by Cu 2+ are 2.2 and 8.0 × 10 −3 M respectively.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
