Abstract
has been studied in fed-batch bioprocesses witha manual (off-line) methanol concentration control [4].These studies demonstrated that variations of the residualmethanol concentration influence drastically in the spe-cific consumption and production rates. To avoid thisproblem a predictive control algorithm coupled with a PIfeedback controller has been satisfactorily implemented[5].This set-up has allowed for further analysis of several keyparameters influencing heterologous protein productionin
Highlights
An important number of heterologous proteins have been produced in the methylotrophic yeast Pichia pastoris using the methanol-inducible alcohol oxidase promoter [1]
When the methanol set point was set at 0.5 g· L-1, the qp reached a maximum of 340 UA· gX-1· h-1 at the beginning of the induction phase, followed by a sharp decrease to almost zero values after 20 h of induction
Microbial Cell Factories 2006, 5(Suppl 1):S13 batch cultivation carried out at a set point of 1 g· L-1, the lipolytic activity values remained very low during a considerable period (20 h of induction phase); after this lag phase, a maximum qp value of 440 UA· gX-1· h-1 is reached after 40 h of induction phase, which was followed by an exponential decrease to values below 100 UA· gX1· h-1 after 75 h of fed-batch phase
Summary
An important number of heterologous proteins have been produced in the methylotrophic yeast Pichia pastoris using the methanol-inducible alcohol oxidase promoter [1]. Substrate feeding strategies in Pichia pastoris fed-batch cultivation processes: Analysis of key parameters influencing recombinant protein production
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