Abstract
The key results of a theoretical model of immobilized-enzyme reactors and enzyme electrodes are summarized, and the applicability of the theory is discussed. The model was used to analyze the performance of a miniaturized urease reactor (coupled to an ionselective electrode cell). For the freshly prepared reactor a substrate-conversion efficiency of ∼100% was established. Aging of the reactor was quantitatively studied by fitting theoretical curves to experimental response data and evaluation of the kinetic parameters. The life time turned out to be limited by an exponential decay of enzyme activity.
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