Abstract
Within the ruminant system, several possibilities exist to generate dendritic cells migrating out from the tissue into the regional draining lymph nodes as afferent lymph dendritic cells (ALDCs). Here, we analyzed toll-like receptor (TLR) 1–10 mRNA expression by using quantitative real-time PCR in highly purified subsets of bovine ALDC. As TLR expression may be influenced by pathogens or vaccines and their adjuvant, it is necessary to understand what TLRs are expressed in a steady-state system to elucidate specific differences and to potentially optimize targeted vaccines. In this study, we have assessed the TLR expression profiles of the four main bovine ALDC subsets [cDC1 and cDC2 (subsets 2–4)]. We demonstrate differences in TLR expression between the four subsets that may reflect the ability of these cells to respond to different pathogens or to respond to adjuvants.
Highlights
Dendritic cells (DC) play a crucial role in the immune response; they are the key antigen-presenting cell (APC) orchestrating adaptive immune responses and are important in effecting potent T-cell responses
We demonstrated that differences exist between the toll-like receptor (TLR) expression profiles of bovine monocytes, monocyte-derived macrophages, alveolar macrophages, monocyte-derived DC, bone marrow-derived DC, and CD172a+ and CD172a− subpopulations of afferent lymph dendritic cells (ALDCs) [20]
A detailed transcriptome analysis has not been performed for bovine ALDC, and the expression profile of TLRs on these cells has not been described
Summary
Dendritic cells (DC) play a crucial role in the immune response; they are the key antigen-presenting cell (APC) orchestrating adaptive immune responses and are important in effecting potent T-cell responses. We demonstrated that differences exist between the TLR expression profiles of bovine monocytes, monocyte-derived macrophages, alveolar macrophages, monocyte-derived DC, bone marrow-derived DC, and CD172a+ (cDC2) and CD172a− (cDC1) subpopulations of ALDC [20]. To date, quantitative measurement of TLR expression by specific subsets of bovine ALDC has not been performed.
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