Abstract
A combination of E-rosetting techniques with both neuraminidase- and AET-treated sheep erythrocytes (RBC) was used to enumerate subsets of bovine T lymphocytes. Direct (anti-Ig) rosetting procedures were used to enumerate B lymphocytes bearing surface immunoglobulin (Ig). Approximately 10% of bovine peripheral blood lymphocytes formed rosettes only with neuraminidase-treated RBC; 20% formed rosettes with either neuraminidase- or AET-treated RBC; 30% formed rosettes only with AET-treated RBC; 25% possessed surface Ig, as shown by rosette formation with anti-Ig-coupled RBC; and the remaining 15% lacked both E receptors and surface Ig. These five populations were physically separated by centrifugation on Ficoll-Diatrizoate and recovered for functional analysis. The procedures reported here should be useful for the identification of lymphocyte populations responsible for recognition, effector, and cooperative functions in the bovine immune system.
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