Abstract

A new quantitative submicroassay, based on the ferric acetate reagent system, is described and successfully applied to the analysis of cholesterol in 1-mm diameter, 16-μm thick, single microtome sections of adrenal gland, without requiring prior solvent extraction. The method employs 1 100th of the sample size required in the original method, and is also more sensitive than the quantitative histochemical methods that are currently available. The method permits accurate determination of 0.05–3.0 μg amounts of cholesterol in submicroliter volumes of biological fluids or microgram amounts of frozen-dried tissue.

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