Submerged fermentation of wheat bran by Aspergillus flavus for production and characterization of carboxy methyl cellulase

Abstract

ObjectiveTo elucidate the capabilities of carboxy methyl cellulase (CMCase) production with the selective species of Aspergillus flavus (A. flavus). MethodsThe carboxy methyl cellulase (CMCase) expression in A. flavus was evaluated under different environmental conditions using submerged fermentation (SmF) on different agricultural byproducts. ResultsA. flavus produced high levels of CMCase under optimized culture conditions on 3rd day of incubation at an optimum pH 6.0, temperature 30 °C, inoculum size 4% in Czapek Dox using wheat bran as a substrate by SmF. Enhanced production occurred on addition of 4% wheat bran and 1% peptone as nutritional factors. The enzyme hydrolyzed wheat bran exhibiting Vmax value of 40 U/mg and K m value of 0.5 mg/mL at pH of 7. The enzyme was optimally activated 50 °C. Except Mg2+, all other metal ions such as Zn2+, Cu2+, Na+ and Ca2+ were found to be activators for the enzyme activity. ConclusionThe kinetic parameters showed the thermo stability, pH stability of the enzyme which reflects the potential and commercial significance of the enzyme.