Abstract
Rainbow trout were injected intraperitoneally with 40 or 80mg kg-1 body weight of the commercial PCB (polychlorinated biphenyl) mixture Clophen A50. Five weeks after the Clophen A50 injection, the fish were immunised with keyhole limpet haemocyanin (KLH). Another 4 weeks later, the fish were sacrificed and samples were taken for immunological, haematological, chemical and histological analysis. Histological changes in the thymus and the spleen due to Clophen A50 exposure included oedema and partial degeneration of the lymphoid tissue. In addition, lipoid degeneration of hepatocytes and bile duct hyperplasia were observed in livers from exposed fish. Plasma electrolytes and enzyme levels, blood leucocyte counts or antibody levels to KLH were not altered by exposure to Clophen A50. The responsiveness of splenocytes to both T and B cell mitogens (lipopolysaccharide and phytohaemagglutinin) increased significantly in the PCB-exposed fish after immunisation with KLH. Such effects were not observed in non-exposed fish. These findings indicate that Clophen A50 exposure may affect cellular immune functions in rainbow trout.
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