Abstract
Prerequisites and objective: Abdominal obesity is a common metabolic disease characterized by excessive accumulation of white adipose tissue, with excessive production of inflammatory mediators that activate transcriptional nuclear factors, in particular the nuclear factor kB. Hepcidin is the main hormone in the systemic regulation of iron. Its expression increases with elevated level of pro-inflammatory cytokines, as a result of which the concentration of iron in the blood plasma decreases, which reliably results in the activation of NF-kB. Therefore, the aim of the research was to determine the level of expression of ІКβα in subcutaneous adipose tissue in iron deficiency anemia concurrent with obesity. Methods: The study included 40 female women. Hemogram parameters, parameters of iron metabolism (serum iron, ferritin, hepcidin, total iron binding ability of serum, saturation of transferrin by iron) in serum and level of expression of the IkBα gene in subcutaneous adipose tissue were evaluated. Research results. The examined women had an average age of 40.3 ± 7.59 years. In the distribution of patients due to the cause and degree of severity of IDA, there was no reliable difference between the groups. The control group consisted of 10 obese women without IDA. Expression of IkBα by subcutaneous adipose tissue in female patients with IDA with and without obesity compared with the control group did not display a significant difference between the groups (p <0.05). In the course of correlation analysis, the expression of IkBα by subcutaneous adipose tissue to BMI, iron and hemogram rates did not have a correlated significance. Conclusion. Subcutaneous adipose tissue in women with iron deficiency anemia with and without obesity compared with obese women has no difference in expression of IkBα.
Highlights
Abdominal obesity is a common metabolic disease characterized by excessive accumulation of white adipose tissue.Subcutaneous adipose tissue is infiltrated by numerous immune cells, primarily macrophages, which account for up to 40% of all fatty tissue cells [1] and are the main source of inflammatory mediators in the fatty tissues of humans and animals
The activation of nuclear factor kB (NF-kB) may be evidenced by changes in the expression of its inhibitor, IkBα [6], the aim of our study was to determine the level of expression of ІКβα in subcutaneous adipose tissue in iron deficiency anemia concurrent with obesity
In the analysis of anthropometric data of women, the group with iron deficiency anemia (IDA) and obesity had statistically higher body mass index (BMI) compared to the group with IDA and without obesity (p 0.05)
Summary
Subcutaneous adipose tissue is infiltrated by numerous immune cells, primarily macrophages, which account for up to 40% of all fatty tissue cells [1] and are the main source of inflammatory mediators in the fatty tissues of humans and animals. Iron plays an important role in immunological surveillance due to its stimulating and differentiating properties on the immune system. Excessive concentration of iron in macrophages reduced the level of phagocytosis, respiratory burst and cytokine expression – the effects that are probably mediated by iron toxicity. In non-toxic concentrations, iron contributes to the differentiation of macrophages, and treatment with iron chelators inhibits the expression of NF-KB activated by lipopolysaccharides, tumor necrosis factor-α and interleukin-6 [3]. The addition of ionized iron to the macrophage culture of the liver caused activation of IKK (a complex that is a critical point of this signaling pathway) [4], which con-
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
