Subclinical endometritis and postpartum ovarian resumption in respect to TNF-α, IL-8 and CRP in Egyptian buffaloes.

Doaa H Elsayed,Eman A Ahmed,Sherif M Dessouki,Yasmina K Mahmoud,Fakhri E El-Azzazi

doi:10.21451/1984-3143-ar2019-0027

Abstract

The study was carried out to find the relation between subclinical endometritis (SCE) and postpartum (pp.) ovarian resumption as well as to evaluate serum and endometrial TNF-α, IL-8 and serum CRP in buffaloes with and without SCE. Thirty-nine pluriparous buffaloes at the 3rd (W3), 5th (W5) and 7th (W7) week pp. were involved in this experiment. The parity of the buffaloes ranged from 4 to 8 with an average 5.8±0.2. Subclinical endometritis was diagnosed by the percentage of polymorphonuclear cells (PMNs) in uterine cytology obtained from endometrial cytobrush at W5 and W7. The cut-off point of PMNs% in buffaloes for SCE was ≥ 6% at W5 or ≥ 4% at W7. According to PMNs%, buffaloes were divided into SCE group (n=27) and non-SCE group (n=12). Ovarian cyclicity was monitored by rectal palpation, ultrasonography and progesterone assay at W3, W5 and W7. Serum and endometrial TNF-α, IL-8 and serum CRP were estimated at W5 and W7. Buffaloes with SCE (55.6%) showed delayed ovarian activity as compared to non-SCE (16.7%) animals (P=0.036). Significant increase in serum cytokines and CRP levels were detected at W5 (P ˂0.05) and W7 (P <0.01) in SCE buffaloes as compared to non-SCE. Endometrial levels of cytokines were significantly (P ˂0.05) elevated in SCE buffaloes. Serum and endometrial cytokines showed significant positive correlation. Furthermore, levels of TNF-α, IL-8 and CRP exhibited significant positive correlation with PMNs%. In conclusion, SCE delayed postpartum ovarian cyclicity in buffaloes. Moreover, TNF-α, IL-8 and CRP assessments could be efficient tools in prediction of SCE in buffaloes.

Highlights

Identification and recognition of biomarkers help in early diagnosis and assessment of the animal’s pathophysiological condition of some diseases (Manimaran et al, 2016)
Asymptomatic endometritis known as subclinical endometritis (SCE) or cytological endometritis (Boby et al, 2017; Ghanem et al, 2015)
The postpartum period is critically substantial for fertility and reproductive performance of buffaloes

Summary

Introduction

Identification and recognition of biomarkers help in early diagnosis and assessment of the animal’s pathophysiological condition of some diseases (Manimaran et al, 2016) Among these diseases is, asymptomatic endometritis known as subclinical endometritis (SCE) or cytological endometritis (Boby et al, 2017; Ghanem et al, 2015). Buffaloes suffered from SCE showed increased days to first estrus, prolonged days open (Ali et al, 2009), repeat breeding, reduced conception and pregnancy rates (Senosy and Hussein, 2013). These impairments in fertility (Singh et al, 2018) as well as reproductive performance gave rise to threats of culling and economic losses (Pande et al, 2013).

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Conclusion