Subclasses of porcine immunoglobulins G. Production of subclass specific antisera with S-sulfonated gamma chains.

Abstract

AbstractUsing antisera made against porcine S‐sulfonated heavy chains two subclasses of porcine IgG could be distinguished on the basis of antigenic determinants located on the Fc fragment. The antisera prepared against the porcine gamma chains reacted with other mammalian IgG proteins, but not with avian, amphibian or fish immunoglobulins. Antigenic determinants responsible for cross‐reactivity are further localized on the Fc′ fragment of the porcine IgG molecule. Comparable antisera, specific for porcine light chains, did not cross‐react with IgG from any of the other species tested. Porcine IgG and F(ab′)2 fragment reacted with both the anti‐heavy and anti‐light chain sera, while the Fab fragment reacted only with the anti‐light chain sera. Important antigenic determinants are thus located on the light chains and the Fc portion of the heavy chain of the porcine IgG molecule.