Sub-chronic effects of a phosphorothionate insecticide (RPR-II) on hemopoietic system and serum composition of male and female rats

Abstract

The effect of RPR-II (a novel phosphorothionate) was studied in blood hematology and clinical biochemistry parameters in male and female rats. They were orally given sub-chronic doses of 0.014 (low), 0.028 (medium) and 0.042 (high) mg kg−1 for a period of 90 days. Results showed that RPR-II caused a significant (P<0.05) decreased body weight gain, feed intake, hemoglobin (Hb), hematocrit (Hct) and total erythrocyte (TEC) whereas total leukocyte (TLC), absolute values of mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) increased significantly. Similarly, due to the sub-chronic treatment of RPR-II the clinical blood biochemistry parameters like calcium, phosphorus, creatinine and chloride increased significantly (P<0.05) whereas, protein and glucose contents decreased in male- and female-treated rats after 45 and 90 days of treatment. These alterations were significant when compared with two-way ANOVA analysis showing that they were dose and time dependent. The alterations in male rats when compared with female rats were statistically insignificant showing no sexual dimorphism by this compound. After 28 days of treatment (withdrawal study) recovery was observed indicating a reversal of the changes once the toxicant is removed. These alterations in hematological and clinical biochemistry parameters show toxic stress in the treated animals especially on blood and blood forming organs.