Abstract
Fluorescent in situ hybridization (FISH) has become one of the most powerful techniques in human cytogenetics. The characterization of chromosomal rearrangements has been greatly facilitated by the availability of several kind of probes suitable for FISH experiments: 1. large inserts accommodated in vectors like phage, cosmid, BAC, YAC etc.; 2. probes recognizing satellite DNAs (alphoid DNA, satellite III etc.); 3. whole chromosome painting libraries (WCPL), obtained from sorted chromosomes (Collins et al., 1991), or from somatic cell hybrids retaining single whole human chromosomes (Lichter et al. 1990; Liu et al. 1993); 4. microdissection libraries, specific for single chromosome bands (Meltzer et al., 1992; Guan et al., 1995); 5. subchromosomal painting libraries (SCPLs), specific for a definite chromosomal region. SCPLs can be generated, via Alu-PCR, from somatic cell hybrids retaining fragments of human chromosomes (Archidiacono et al., 1994).
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