Abstract
Abstract The subcellular representation of thymus-leukemia (TL) antigens in murine cells was studied by inhibition of cytotoxic antiserum. By differential centrifugation, subcellular fractions were obtained from 6 sources: thymocytes of B6 mice (TL-); thymocytes of a congenic mouse line (B6-TL+) differing from B6 at the Tla locus; a TL+ B6 leukemia (ERLD); a TL-B6 leukemia (E♂ G2); and liver cells from B6 and B6-TL+ mice. Only fractions obtained from the phenotypically TL+ sources, i.e., B6-TL+ thymocytes or ERLD leukemia cells contained TL antigen. Plasma membranes were most active, followed by mitochondria and microsomes. Nuclear and cytosol fractions contained no intrinsic activity. These results suggest that the phenotypic expression of TL antigens is not a question of selective expression at the cell surface but reflects instead repression vs derepression at the level of the genome.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
