Abstract
The nuclear-cytoplasmic partition of newly synthesized C, D and 5 S RNAs of HeLa cells was studied with aqueous and non-aqueous methods of cell fractionation. The level of briefly labeled 5 S RNA in cytoplasmic fractions prepared by a non-aqueous method is lower than in cytoplasmic fractions obtained by an aqueous method. The reverse is true in nuclear fractions. At the same time, with both aqueous and non-aqueous cell fractionation, the level of briefly labeled precursors to RNAs C and D is similarly high in cytoplasmic fractions and low in nuclear preparations. This suggests that the previously reported finding that RNA species C and D are cytoplasmic during the first minutes of their lifetime, may represent an in vivo phenomenon, instead of an artifactual elution from nuclei during fractionation.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
