Abstract
1. 1. Rate dependent and isopycnic banding in a zonal rotor were used to analyse the subcellular sites of enzymes in homogenates of guinea-pig small intestinal mucosa. 2. 2. The results demonstrate the following localizations: monoglyceride acyltransferase—microsomal; xanthine oxidase and dehydrogenase—soluble phase, and NADP: isocitrate dehydrogenase—soluble phase and mitochondrial. 3. 3. Alkaline phosphatase is confined to brush borders and is absent from the basolateral plasma membrane. A variable proportion of the activity, up to 40% is on brush borders which during homogenization break up into particles of reduced density and slow sedimentation rate.
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