Abstract
Subcellular localization of superoxide dismutase (SOD) isozymes in Chlamydomonas reinhardtii Dangeard was investigated. From both biochemical and immunological studies, Fe‐ containing superoxide dismutase (Fe SOD) was localized in the chloroplast, and the major Mn‐containing SOD (Mn SOD) was localized in the mitochondria. We isolated cDNA clones, Sod1, and Sod2, which encode Mn SOD and Fe SOD, respectively. Genomic DNA hybridization with these SOD clones demonstrated that there are two additional sequences closely related to Sod1. The transcriptional levels of both genes were compared in light‐ and dark‐grown cells. Mn SOD was higher in heterotrophically grown cells, whereas the accumulation of Fe SOD transcripts was higher under phototrophic condition.
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