Abstract
Cellular uptake and subcellular localization of daunorubicin were studied in Ehrlich ascites tumor cells, either in vivo after inoculation of the drug to mice or in vitro after incubation of cultured Ehrlich ascites tumor cells with the drug. These cells were either sensitive (wild-type) or resistant to daunorubicin. The uptake of daunorubicin was 2–4 times less in resistant cells. About 90% of the drug which had penetrated in the cells was recovered in the nuclei. By cell fractionation, it has been established that the extranuclear daunorubicin was localized in the lysosomes. The lower uptake of the drug in resistant cells and the similar subcellular localization in both types of cells were in favour of an increased active outward transport of the drug in resistant cells.
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