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Abstract
Classical fractionation studies showed that xanthine dehydrogenase (EC 1.2.1.37) was exclusively cytosolic in chicken liver. Fumarase (EC 4.2.1.2) and malate dehydrogenase (EC 1.1.1.37) were also found to have major cytosolic locations. These data indicate that urate synthesis in chicken liver produces substantial quantities of cytoplasmic NADH which may supply reducing equivalents of gluconeogenesis and other processes.
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