Abstract

It has long been known that a-tocopherol occurs in plant tissue. Since tocopherol in animal tissue has been found in mitochondria and chromanol structures have been proposed as intermediates in oxidative phosphorylation (6, 4) it was of interest to determine whether tocopherol is found in mitochondria and chloroplasts of plant cells. The occurrence of 'tocopherol in the organelles of plant cells would provide new systems in which to consider its functional significance. To establish the intracellular distribution of tocopherol in leaf tissue we have made an extensive study of the subcellular fractions of spinach leaf as well as a study of lilac leaf tissue. The results indicate that a-tocopherol is primarily localized within the chloroplast structure, but with an amount also in the mitochondria and in less dense structures or soluble material of the fraction not sedimented at 78,000 X g.

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