Subcellular distribution of phytin in the endosperm of developing castor bean: a possibility for its synthesis in the cytoplasm prior to deposition within protein bodies

Abstract

Studies using light and electron microscopy, and energy-dispersive X-ray analysis have allowed us to identify phytin particles within the cytoplasm of the developing endosperm of castor bean (Ricinus communis L.). These particles are present at the time of the formation of globoid particles within the protein bodies, but they are absent from mature tissue with fully formed protein bodies. We suggest that phytin is formed initially in the cytoplasm (perhaps in association with the cisternal endoplasmic reticulum) before being transported to the protein bodies, wherein it condenses to form the globoid.