Abstract
Palmitoyl-CoA hydrolase releases free fatty acids which are regarded as precursors of metabolites giving off-flavour in unblanched frozen carrot cubes. The subcellular distribution of the enzyme has been studied by differential centrifugation using marker enzymes. Palmitoyl-CoA hydrolase activity was mainly found in the cytosol, but a membrane bound endoplasmatic and mitochondrial localization was also found. The importance of the enzyme during cell disruption is discussed.
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