Abstract
A method is described for the measurement of androgens in subcellular fractions of the human prostate. The reliability criteria compare favorably to those of methods described for measurement of androgens in human prostate homogenates. This study shows that the larger fraction of cellular DHT is localized in the nucleus, only 25–30% being found in the cytosol. The concentration of androgens in cytosol is of the same order as the sum of receptor binding sites and TeBG binding sites described in the literature. As to the androgen concentration in the nuclei, we found a mean DHT concentration of 1125 pmol/100 μg DNA, significantly higher than the receptor concentration in the nucleus, suggesting that a large fraction of nuclear androgen is not bound to the receptor.
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