Subcellular Distribution and Chemical Forms of Heavy Metals in Three Types of Compositae Plants from Lead-Zinc Tailings Area

Abstract

Field investigation on the content of heavy metals in soils and three types of widely distributing compositae plants(Artemisia lavandulaefolia, Ageratum conyzoides L., Crassocephalum crepidioides) in lead-zinc tailings farmland of Yangshuo, Guangxi Zhuang Autonomous Region was carried out, and the differential centrifugation technique and sequential chemical extraction method were used to study the subcellular distribution and chemical forms of heavy metals in these plants. The results indicated that the soil in the tailings farmland was highly contaminated by Cd, Pb and Zn, and their concentrations were 37.7, 5.7 and 8.9 times higher than their respective values of national standard for soil environment quality(GradeⅡ). The contents of Cd, Pb and Zn in the analyzed plants exceeded the normal ranges. Ageratum conyzoides L. and Crassocephalum crepidioides showed strong capability in tolerance, accumulation and transport of Cd, and they could be used as pioneer plants for Cd-phytoremediation in study area and some related areas. In addition, Cd concentrations in the stem and leave of Crassocephalum crepidioides were 159.6 mg·kg-1 and 219.5 mg·kg-1, respectively, which exceeded the threshold of Cd hyperaccumulator. Thus, Crassocephalum crepidioides can be regarded as a Cd-hyperaccumulator. Majority of total Cd, Pb and Zn were found in soluble fraction and cell wall in the plants, while only a small quantity were distributed in organelles. As for chemical forms distribution, Cd, Zn and Pb predominated NaCl-, HAC-, and HCl-extractable forms both in roots and shoots of the plants. Therefore, cell wall binding, vacuolar compartmentalization and distribution mainly in lower active chemical forms were supposed to be the main mechanisms for tolerance to heavy metals in the study plants. A difference of Cd subcellular distribution and its chemical forms in the three Compositae plants was observed. Compared with the shoot of Artemisia lavandulaefolia, more Cd was located in the vacuolus and cellular soluble parts in Ageratum conyzoides L. and Crassocephalum crepidioides. Moreover, the proportion of active chemical Cd in the shoots was lower than that in the roots. These results showed that the subcellular distribution and chemical forms of Cd were related to the plant species,and also indicated Ageratum conyzoides L. and Crassocephalum crepidioides were likely to possess a higher tolerance and accumulation of Cd.