Abstract
Nanopatterning of proteins is important for many bio-diagnostic applications. We report on 200-nm scale patterning of thin films of proteins, by a specifically designed soft lithographic technique. This employs an elastomeric replica of a master, whose patterned side is firstly spin-coated by the protein and then turned upside down on a glass substrate. The patterned protein film is completely transferred to the substrate, exploiting the hydrophobic character of the elastomer. The process completely preserves the properties of biological matter, as it operates at room temperature and it does not require any bio-destructive solvent.
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