Abstract
The aim of this study was to examine hepatotoxicity of Plantago mayor L. extract on rat by using effective dosage. By experimental study and post test only with control group design. 15 rats (Rattus norvegicus) were divided in to 3 group. Group A as a natural control was given aquades. Group B and C were given extract of Plantago major L. 50mg, and 100mg/200g BW rat/day per oral. Liver function was determined with measurement of Aspartate aminotransferase (AST) level, Alanine aminotransferase (ALT), total bilirubin, and histopathological feature of liver. Blood sampling and Liver organ were taken after 28 days of intervention. The average of AST levels, ALT and total bilirubin among groups A, B and C were AST levels (145.40±52.92, 129.00±34.89, and 115.60±13.24 U/l), ALT (76.40±18.87, 83.20±18.71, and 61.00±8.45 U/l) and total bilirubin (0.56±0.03, 0.77±0.22, and 0.58±0.08 mg/dL). Statistical analysis showed that there were not significantly differences of AST levels (p=0,63; CI95%), ALT (p=0,47; CI95%) and total bilirubin (p=0,0,09; CI95%) between the groups. In histopathological features, the average Scheuer score between groups A, B and C is 1.79 ± 0.74, 3.30 ± 0.66 and 2.84 ± 0.77. There is a significant difference in Scheuer scores between the groups (p=0,005; CI95%) that show that there is a difference in the effect of giving extract of Plantago major L. to hepatocyte cells leading to a piecemeal necrosis. This study can be concluded that in effective dosage, Plantago major L. extract able to induce hepatocytes injury although it cannot cause liver disfunction yet.
Highlights
Plantago major L. is a weed that grows in many tropical regions, include Indonesia both in the highlands and in the lowlands
Other studies have shown that ethanol extract of Plantago Major L. can prevent gastric ulceration and inhibit the growth of Helicobacter pylori in vitro so that it is potentially developed as a gastrointestinal disorder drug, such as dyspepsia, gastritis to peptic ulcer (Cogo et al, 2010; Awaad, El-Meligy, & Soliman, 2013)
The differences in levels of AST and ALT that were not significant between the control group and the study group showed that Plantago major L. extract didnot cause severe toxic effects on the liver
Summary
Plantago major L. is a weed that grows in many tropical regions, include Indonesia both in the highlands and in the lowlands. This plant has many names depending on the area where it grows. Other studies have shown that ethanol extract of Plantago Major L. can prevent gastric ulceration and inhibit the growth of Helicobacter pylori in vitro so that it is potentially developed as a gastrointestinal disorder drug, such as dyspepsia, gastritis to peptic ulcer (Cogo et al, 2010; Awaad, El-Meligy, & Soliman, 2013). In studies related to chemopreventive effects, ethanol extract of Plantago Major L. can inhibit excessive expression of the Regenerating-1α gene responsible for gastric carcinogenesis and increase Caspase-3 which can increase cancer cell apoptosis. The most effective dosage of Plantago major L. extract found in this research were 100 mg/200g BW rat (Sutrisna, Ani, Muchtan, & Herri, 2013).
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
