Abstract
A 5-year old Holstein Friesian cross breed cow was presented to Madras Veterinary College Teaching Hospital with the history of reduced milk yield. Clinical examination of udder revealed normal milk color and soft udder. The milk pH was 7.0, with California Mastitis Test score 3+, Electrical Conductivity 270U, and Somatic Cell Count as 328,000. Isolation and identification of causative agent revealed Methicillin Resistant Staphylococcus aureus (MRSA) from the sub-acute mastitis sample. Agar disc diffusion method for antimicrobial susceptibility revealed that the MRSA was sensitive to Enrofloxacin, Gentamicin, Oxytetracycline and Amoxicillin+Sulbactam. On the other hand, the isolate was resistance to Amoxicillin, Penicillin G, Ceftriaxone and Methicillin. The isolate was positive for β-lactamase resistance by Nitrocefin test. The MRSA was confirmed for the presence of mecA and blaZ target genes by polymerase chain reaction (PCR). The cow was treated with Enrofloxacin, Vitamin E and inorganic Selenium, and was recovered after 5 days of post-treatment.
Highlights
A 5-year old Holstein Friesian cross breed cow was presented to Madras Veterinary College Teaching Hospital with the history of reduced milk yield
The Staphylococcus isolate was reconfirmed as Methicillin Resistant Staphylococcus aureus (MRSA) by amplifying the MRSA specific genes using the gene specific primers, as described by Lee (2003) and Martineau et al (2000), respectively (Figure 1, 2)
The milk pH was 7.0, California Mastitis Test (CMT) result was 3+, Electrical Conductivity (EC) was 270U, and SSC was 328,000; these were suggestive for being the case as sub-clinical mastitis
Summary
A 5-year old Holstein Friesian cross breed cow was presented to Madras Veterinary College Teaching Hospital with the history of reduced milk yield. BlaZ, Bovine mastitis, mecA, PCR, Staphylococcus aureus The animal was suspected to be infected with sub-clinical mastitis. The MRSA commercial kit (MRSA AlertTM Kit, HiMedia, India) was used for confirmation of Methicillin Resistant Staphylococcus aureus (MRSA) following the instructions of the manufacturer, and presence of a clot and color change in the medium from red to yellow after 24 h (Selepak and Witebsky, 1985; Begum et al, 2007).
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