Abstract
DCLK1) or green fluorescent protein (GFP) cDNA sequence as control (AsPC-GFP). Gene expressing levels in both AsPC-DCLK1 and AsPC-GFP cells were analyzed by real-time RTPCR. The proliferative and invasive potential of these cells were compared using a MTT assay for proliferation, wound healing assay for migration, soft agar assay for clonogenicity, andMatrigel coated transwell assay for invasion.Results:DCLK1mRNA levels were increased more than 50 fold in AsPC-DCLK1 cells. Compared to AsPC-GFP cells, AsPC-DCLK1 cells exhibited a more than 20% increase in proliferation, approximately 30% increase in colony formation, 20% increase in migration, and a 2-fold increase (p < 0.05) in invasion. Conclusion: These data taken together suggest that DCLK1 promotes pancreatic cancer cell EMT and tumorigenesis, this propertymay contribute to pancreatic cancer metastasis and resistance to standard chemotherapy. Thus, these findings support the development of anti-DCLK1 based therapeutics to target CSCs for PDAC treatment.
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