Su1811 Sitagliptin Can Be Anti-Inflammatory Drug for the Development of NAFLD in Obese Diabetic OB/OB Mice Under Lipopolysaccharides Existence

Abstract

the biopsy day. Serum miR-34a was analyzed by quantitative real time PCR method (Applied BiosynthesisTM) and expressed as copies/μL. Results: The mean age of NAFLD patients was 47.1 ± 13.6 year with female of 56%. Their mean body mass index (BMI) was 32.3 ± 16.4 kg/m2. Obesity was found in 74.4%, and metabolic syndrome was 76.4%. Liver histopathology showed that 55.8% of patients had NAS ≥4 and the remaining of 44.2% had NAS <4. Two-third of patients (67.4%) had histopathology compatible with NASH and significant fibrosis ( ≥ F2) was found in 25.6%. Serum level of miR-34a showed significant correlation with NAS (r = 0.44, P = 0.003) (Figure 1), degree of steatosis (r = 0.34, P = 0.026), degree of ballooning (r = 0.31, P = 0.043), and degree of fibrosis (r = 0.34, P = 0.002), whereas miR-34a was not correlated with the degree of lobular inflammation (r = 0.21, P = 0.178). Interestingly, serum miR-34a in patients with NAS ≥4 was significantly higher than those with NAS <4 (338.9 ± 353.2 vs 118.4 ± 154.9, P = 0.01) (Figure 2). There was no significant correlation between serum miR-34a and the other variables including age, body weight, height, and BMI. Conclusion: Serum level of microRNA-34a had significantly fair to good correlation with NAS and degree of fibrosis which represent the severity of inflammation. Additionally, serum miR-34a level in patients with NAS ≥4 was significantly higher than those with NAS <4. Thus, microRNA-34a may serve as a potential biomarker of liver inflammation and fibrosis in NAFLD patients.